SUPERDEX 75 10/300 GL
产品名称： SUPERDEX 75 10/300 GL
英文名称： SUPERDEX 75 10/300 GL
产品编号： 17-5174-01/28-9205-04
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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| 17-5172-01 | SUPEROSE 6 10/300 GL |
| 17-5173-01 | SUPEROSE 12 10/300 GL |
| 17-5174-01 | SUPERDEX 75 10/300 GL |
| 17-5175-01 | SUPERDEX 200 10/300 GL |
| 17-5176-01 | SUPERDEX PEPTIDE 10/300 GL |
| 28-9065-61 | Superdex 200 5_150 GL |
| 28-9205-04 | Superdex 75 5_150 GL |
| 17-0506-01 | PP COLUMN MONO Q HR 16/10 |
| 17-0507-01 | PP COLUMN MONO S HR 16/10 |
| 17-1002-01 | MONO Q 60/100 |
| 17-1177-01 | RESOURCE Q, 1 ML |
| 17-1178-01 | RESOURCE S, 1 ML |
| 17-1179-01 | RESOURCE Q, 6 ML |
| 17-1180-01 | RESOURCE S, 6 ML |
| 17-1184-01 | RESOURCE ETH 1 ML |
| 17-1185-01 | RESOURCE ISO 1 ML |
| 17-1186-01 | RESOURCE PHE 1 ML |
| 17-1187-01 | RESOURCE HIC TEST KIT |
| 17-5166-01 | MONO Q 5/50 GL |
| 17-5167-01 | MONO Q 10/100 GL |
| 17-5168-01 | MONO S 5/50 GL |
| 17-5169-01 | MONO S 10/100 GL |
| 17-5170-01 | MONO P 5/50 GL |
| 17-5171-01 | MONO P 5/200 GL |
| 17-5177-01 | MINI Q 4.6/50 PE |
| 17-5178-01 | MINI S 4.6/50 PE |
| 17-5179-01 | MONO Q 4.6/100 PE |
| 17-5180-01 | MONO S 4.6/100 PE |
| 17-5181-01 | SOURCE 15Q 4.6/100 PE |
| 17-5182-01 | SOURCE 15S 4.6/100 PE |
| 17-5186-01 | SOURCE 15PHE 4.6/100 PE |
| 18-4271-00 | O RING 64.5X3 EPDM GLASS TUBE |
| 18-4284-01 | FILTER TOOL, 60 MM |
| 18-4610-60 | DISTRIBUTION UNIT 60 MM TI 1 |
| 18-4610-62 | O RING 55X1.6 EPDM |
Superdex™ 75 / Superdex™ 200 Columns
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Ideal for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- For rapid protein homogeneity analysis or purity check use the 5/150 GL short columns
Superdex™ 75 / Superdex™ 200 Columns
Technical Information
Superdex prepacked columns are designed for high-performance, laboratory-scale separations of proteins, peptides, and other biomolecules according to size. High performance gel filtration offers fast separations with high resolution for a variety of applications including protein purification, studies of complex formation, and screening of uncharacterized samples.
Superdex 75 5/150 GL and Superdex 200 5/150 GL have small bed volumes, which is useful for a range of applications such as screening solubilization conditions for membrane proteins, the study of protein-protein interactions, and rapid purity check of proteins. These columns are an excellent choice when time and sample/buffer consumption is more important than the highest resolution since less buffer and sample volumes are required.
Superdex columns 10/300 GL and 5/150 GL are prepacked Tricorn columns, see Tricorn™ Column Family, that provide excellent reproducibility and durability in purification using a chromatography system.
| TECHNICAL SPECIFICATIONS | |
| Superdex™ | |
| Exclusion limit (Mr) | |
| Superdex™ 75 | 1 × 105 globular protein |
| Superdex™ 200 | 1.3 × 106 globular protein |
| Separation range (Mr) | |
| Superdex™ 75 | 3000-70 000 globular protein |
| Superdex™ 200 | 10 000-600 000 globular protein |
| Matrix | Spherical composite of cross-linked agarose and dextran |
| Average particle size | 13 μm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place) |
| pH stability | 3-12 (working and long term), 1-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Superdex™ 10/300 GL Columns (Tricorn)* | |
| Bed dimensions | 10 × 300 mm |
| Recommended sample volume | 25-250 μl |
| Bed volume | 24 ml |
| Max. pressure | |
| Superdex™ 75 | 18 bar (261 psi, 1.8 MPa) |
| Superdex™ 200 | 15 bar (217 psi, 1.5 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 1.5 ml/min |
| Superdex™ 200 | 1.0 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Superdex™ PC 3.2/30 Columns | |
| Bed dimensions | 3.2 × 300 mm |
| Bed volume | 2.4 ml |
| Recommended sample volume | 2-25 μl |
| Max. pressure | |
| Superdex™ 75 | 24 bar (348 psi, 2.4 MPa) |
| Superdex™ 200 | 12 bar (174 psi, 1.2 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 0.100 ml/min |
| Superdex™ 200 | 0.100 ml/min |
| Theoretical Plates | > 30 000 m-1 |
| Superdex™ 5/150 column (Tricorn™) | |
| Bed volume | 3 ml |
| Bed dimensions | 5 × 150 mm |
| Recommended sample volume | 4 - 50 μl |
| Recommended flow rate | 0.15 - 0.6 ml/min |
| Max. pressure | |
| Superdex 75 | 1.8 MPa |
| Superdex 200 | 1.5 MPa |
| Max. flow rate (H20 at 25°C) | |
| Superdex 75 | 0.7 ml/min |
| Superdex 200 | 0.8 ml/min |
| Theoretical plates | > 25000 m-1 |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
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)
Separation of standard proteins on Tricorn™ Superdex™ 200.
)
Separation of monomer and dimer on Tricorn™ Superdex™ 75.
)
Column performance test with a set of standard proteins on Superdex™ 75
5/150 GL.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165422