FITC标记的人乳头瘤病毒16型L2抗体
产品名称： FITC标记的人乳头瘤病毒16型L2抗体
英文名称： Anti-HPV16 L2/FITC
产品编号： HZ-8547R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： IF=1:50-200
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Rabbit Anti-HPV16 L2/FITC Conjugated antibody
FITC标记的人乳头瘤病毒16型L2抗体
| 产品编号 | bs-8547R-FITC |
| 英文名称 | Anti-HPV16 L2/FITC |
| 中文名称 | FITC标记的人乳头瘤病毒16型L2抗体 |
| 别 名 | HPV16 L2; HPV16-L2; HPV-16; HPV-16 capsid; HPV16 capsid protein; HPV16 major capsid protein L2; Human papillomavirus type 16 L2; Human papillomavirus type 16 major capsid protein L2; Major capsid L2 protein; Major capsid protein; Major capsid protein L2; VL2_HPV16. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 细菌及病毒 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | HPV16 |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from HPV16 L2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Human papillomaviruses, particularly type 16 (designated HPV16), infect the genital tract and may lead to cervical cancer. Protection against HPV16 is thought to be provided by neutralizing antibodies directed to the major caspid protein L1 of HPV16. HPV16 L1 forms the pentameric assembly unit of the viral shell, and the binding of HPV16 L1 to the cell surface without the involvement of minor capsid protein L2 is believed to be the first step of HPV16 infection. The L1-binding domain located near the C-terminus of L2 binds L1 prior to completion of capsid assembly and is required for efficient encapsidation of the viral genome. In addition, the C-terminus of L1 is necessary for both DNA binding and DNA packaging. Expression of the late gene L1 is restricted to the upper layers of the infected epithelium. HPV16 L1 is able to package unrelated plasmid DNA in vitro and deliver the foreign DNA to eukaryotic cells with the subsequent expression of the encoded gene. L1 shows a diffuse nuclear distribution whereas L2 is localized to punctate nuclear regions identified as promonocytic leukemia protein oncogenic domains (PODs). Coexpression of L1 and L2 induces a relocalization of L1 into the PODs. Function: Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, escorts the genomic DNA into the nucleus, in particular by promoting virion endosomal escape. It is involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward the nucleus. Mediates the viral genome import into the nucleus through binding to host importins. Once within the nucleus, L2 localizes viral genomes to PML bodies in order to activate early gene expression for establishment of infection. Later on, promotes late gene expression by interacting with the viral E2 protein and by inhibiting its transcriptional activation functions. During virion assembly, encapsidates the genome by direct interaction with the viral DNA. Subunit: Interacts with major capsid protein L1. Interacts with E2; this interaction inhibits E2 transcriptional activity but not the DNA replication function E2. Interacts with host GADD45GIP1. Interacts with host HSPA8; this interaction is required for L2 nuclear translocation. Interacts with host importins KPNB2 and KPNB3. Forms a complex with importin alpha2-beta1 heterodimers via interaction with the importin alpha2 adapter. Interacts with host DYNLT1; this interaction is essential for virus intracellular transport during entry. Subcellular Location: Virion. Host nucleus. Note=associated with ND10 nuclear bodies. Post-translational modifications: Highly phosphorylated (By similarity). Similarity: Belongs to the papillomaviridae L2 protein family. Database links: UniProtKB/Swiss-Prot: P03107.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
人类乳头状瘤病毒，特别是16型（指定的HPV16）感染生殖道，并可能导致宫颈癌。HPV16的保护被认为是通过中和HPV16的主要casID蛋白L1的中和抗体提供的。HPV16 L1形成病毒壳的五聚体组装单元，并且HPV16 L1与细胞表面的结合而不涉及小衣壳蛋白L2被认为是HPV16感染的第一步。位于L2的C-末端附近的L1结合结构域在完成衣壳组装之前与L1结合，并且需要高效的病毒基因组包封。此外，L1的C-末端对于DNA结合和DNA包装都是必需的。晚期基因L1的表达仅限于感染上皮的上层。HPV16 L1能够在体外包装不相关的质粒DNA，并将外源DNA传递到真核细胞，随后编码基因的表达。L1显示弥漫性核分布，而L2定位于点状核区域，被确定为原核细胞白血病蛋白致癌区（POD）。L1和L2的共表达诱导L1重新定位到荚中。