钙离子转运ATP酶A2(ATP2A2)重组蛋白说明书

Recombinant ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2)  

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Ile315~Met756

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Liver, Pancreas, Lung. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane

protein. Sarcoplasmic reticulum membrane. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation:100mMNaHCO₃, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 78.2kDa

Accurate Molecular Mass: 78kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in 100mMNaHCO₃, 500mM NaCl (pH8.3) to a concentration of0.1-1.0 mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[IDENTIFICATION]

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用