二肽基肽酶9(DPP9)重组蛋白说明书-分析方法-资讯-生物在线

二肽基肽酶9(DPP9)重组蛋白说明书

作者:上海沪震实业有限公司 2023-04-23T00:00 (访问量:168)

二肽基肽酶9(DPP9)重组蛋白说明书

Recombinant Dipeptidyl Peptidase 9 (DPP9)  

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Val505~Leu862

Tags: Two N-terminal Tags, His-tag and GST-tag

Subcellular Location: Cytoplasm. Cytosol. Purity: >90%

Traits: Freeze-dried powder

Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM

EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 70.7kDa

Accurate Molecular Mass: 71kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in  100mM NaHCO3500mM NaCl (pH8.3) to a concentration of0.1-1.0 mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºCfor 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

2022112504473478334

[IDENTIFICATION]

2022112504474111093

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用

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