Is the antibody always to blame?-标准-资讯-生物在线
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Is the antibody always to blame?

作者：Agrisera AB 2020-10-21T00:00 (访问量:1877)

 Is the antibody always to blame? 

In the example blot to the right, the same antibody was used (Anti-BAK1, AS12 1858) but the proteins were extracted with two different buffers. 
Minor adjustments in the buffer components resulted in the improved detection of the target protein - from no band detected at all as seen in the left panel to a band of correct MW visible in the right panel. 

If you are interested to learn more how to optimize your western blot, you are welcome to contact us or request a copy of the Agrisera Western Blot Guide.
 


 

   Western blot using anti-BAK1 antibodies
 

 

 

 

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